repeated measures general linear model sas proc glm Search Results


lms-200  (Sick AG)
90
Sick AG lms-200
Lms 200, supplied by Sick AG, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/repeated+measures+general+linear+model+sas+proc+glm/lms200+series+lidar+sensor/10__1007_slash_s11119___012___9295___0-83-9-13
Average 90 stars, based on 1 article reviews
lms-200 - by Bioz Stars, 2026-09
90/100 stars
  Buy from Supplier
fifth-generation penn state/ncar mesoscale model (revised mm5)  (Meso Scale Diagnostics LLC)
90
Meso Scale Diagnostics LLC fifth-generation penn state/ncar mesoscale model (revised mm5)
Tested WRF physics parametrization configurations. The CONUS physics suite, recommended by NCAR, was chosen for better reproduction of the PRISM-based precipitation and daily maximum and minimum air temperature for an arbitrarily test year, 2009.
Fifth Generation Penn State/Ncar Mesoscale Model (Revised Mm5), supplied by Meso Scale Diagnostics LLC, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/repeated+measures+general+linear+model+sas+proc+glm/fifth+generation+penn+state+ncar+mesoscale+model++mm5+/pmc10539322-13-15-18
Average 90 stars, based on 1 article reviews
fifth-generation penn state/ncar mesoscale model (revised mm5) - by Bioz Stars, 2026-09
90/100 stars
  Buy from Supplier
repeated measures general linear model (glm) analysis  (CH Instruments)
90
CH Instruments repeated measures general linear model (glm) analysis
Tested WRF physics parametrization configurations. The CONUS physics suite, recommended by NCAR, was chosen for better reproduction of the PRISM-based precipitation and daily maximum and minimum air temperature for an arbitrarily test year, 2009.
Repeated Measures General Linear Model (Glm) Analysis, supplied by CH Instruments, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/repeated+measures+general+linear+model+sas+proc+glm/repeated+measures+general+linear+model++glm++analysis/pmc09983368-228-19-11
Average 90 stars, based on 1 article reviews
repeated measures general linear model (glm) analysis - by Bioz Stars, 2026-09
90/100 stars
  Buy from Supplier
opensim model  (OpenSim Ltd)
90
OpenSim Ltd opensim model
Tested WRF physics parametrization configurations. The CONUS physics suite, recommended by NCAR, was chosen for better reproduction of the PRISM-based precipitation and daily maximum and minimum air temperature for an arbitrarily test year, 2009.
Opensim Model, supplied by OpenSim Ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/repeated+measures+general+linear+model+sas+proc+glm/opensim+model/pm33882437-62-3-4
Average 90 stars, based on 1 article reviews
opensim model - by Bioz Stars, 2026-09
90/100 stars
  Buy from Supplier
lrrk2 p g2019s ki mice  (Taconic Biosciences)
93
Taconic Biosciences lrrk2 p g2019s ki mice
(A) Kymographs of axonal EGFP-LC3+ vesicles in PPM1H KO iNeurons transiently expressing CFP (“Mock”) or ARF6 Q67L -CFP. Example AV traces are highlighted. (B) Directionality of AVs in PPM1H KO iNeurons transiently expressing CFP or ARF6 Q67L -CFP. Antero., anterograde; retro., retrograde; bidir., bidirectional; stat., stationary (mean ± SEM; n = 32-34 neurons from 4 independent experiments; ns, not significant, p>0.9652; *p<0.0410; two-way ANOVA with Sidak’s multiple comparisons test). (C-G) Pause number (C) and pause duration (D) of motile AVs, fraction of time paused (E) of all AVs, and directional reversals (F) and Δ run length (G) of motile AVs in PPM1H KO iNeurons transiently expressing CFP or ARF6 Q67L -CFP (mean ± SD for panel C and E; n = 63-95 motile AVs (C, D, F, G) and 77-134 total AVs (E) from 32-34 neurons from 4 independent experiments; ns, not significant, p= 0.638; *p= 0.0396; ***p<0.001; mixed effects model analysis). For panels C and E, scatter plot points indicate the means of four independent experiments. For panels D, F, and G, curve fits were generated using nonlinear regression (two phase decay). (H-I) Model: imbalance between ARF6 and pRABs disrupts axonal AV transport in conditions of <t>LRRK2</t> hyperactivity (H) Under physiologic conditions, ARF6 binds to JIP4, promoting JIP4 binding to dynactin while discouraging the JIP4-kinesin interaction. With active dynein/dynactin and inactive kinesin, AVs undergo smooth retrograde transport in WT neurons. (I) Under pathologic conditions of LRRK2 hyperactivity, increased phosphorylation of RAB GTPases on the AV membrane leads to increased recruitment of JIP4, due to specific binding of JIP4 by RABs in their phosphorylated state. This allows for recruitment and disinhibition of kinesin by JIP4. The presence of active dynein/dynactin and active kinesin on the same organelle results in an unproductive tug-of-war between microtubule-associated motors, leading to non-processive motility and pausing behavior of AVs.
Lrrk2 P G2019s Ki Mice, supplied by Taconic Biosciences, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/repeated+measures+general+linear+model+sas+proc+glm/LRRK2+G2019S+Mouse/bio_rxiv__2022__11__14__516471-180-0-14
Average 93 stars, based on 1 article reviews
lrrk2 p g2019s ki mice - by Bioz Stars, 2026-09
93/100 stars
  Buy from Supplier
fifth generation mesoscale model mm5  (Meso Scale Diagnostics LLC)
90
Meso Scale Diagnostics LLC fifth generation mesoscale model mm5
(A) Kymographs of axonal EGFP-LC3+ vesicles in PPM1H KO iNeurons transiently expressing CFP (“Mock”) or ARF6 Q67L -CFP. Example AV traces are highlighted. (B) Directionality of AVs in PPM1H KO iNeurons transiently expressing CFP or ARF6 Q67L -CFP. Antero., anterograde; retro., retrograde; bidir., bidirectional; stat., stationary (mean ± SEM; n = 32-34 neurons from 4 independent experiments; ns, not significant, p>0.9652; *p<0.0410; two-way ANOVA with Sidak’s multiple comparisons test). (C-G) Pause number (C) and pause duration (D) of motile AVs, fraction of time paused (E) of all AVs, and directional reversals (F) and Δ run length (G) of motile AVs in PPM1H KO iNeurons transiently expressing CFP or ARF6 Q67L -CFP (mean ± SD for panel C and E; n = 63-95 motile AVs (C, D, F, G) and 77-134 total AVs (E) from 32-34 neurons from 4 independent experiments; ns, not significant, p= 0.638; *p= 0.0396; ***p<0.001; mixed effects model analysis). For panels C and E, scatter plot points indicate the means of four independent experiments. For panels D, F, and G, curve fits were generated using nonlinear regression (two phase decay). (H-I) Model: imbalance between ARF6 and pRABs disrupts axonal AV transport in conditions of <t>LRRK2</t> hyperactivity (H) Under physiologic conditions, ARF6 binds to JIP4, promoting JIP4 binding to dynactin while discouraging the JIP4-kinesin interaction. With active dynein/dynactin and inactive kinesin, AVs undergo smooth retrograde transport in WT neurons. (I) Under pathologic conditions of LRRK2 hyperactivity, increased phosphorylation of RAB GTPases on the AV membrane leads to increased recruitment of JIP4, due to specific binding of JIP4 by RABs in their phosphorylated state. This allows for recruitment and disinhibition of kinesin by JIP4. The presence of active dynein/dynactin and active kinesin on the same organelle results in an unproductive tug-of-war between microtubule-associated motors, leading to non-processive motility and pausing behavior of AVs.
Fifth Generation Mesoscale Model Mm5, supplied by Meso Scale Diagnostics LLC, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/repeated+measures+general+linear+model+sas+proc+glm/mm5+model/us09310517-3-9-11
Average 90 stars, based on 1 article reviews
fifth generation mesoscale model mm5 - by Bioz Stars, 2026-09
90/100 stars
  Buy from Supplier
voltage clamp/pulse generator model vcc600  (Physiologic Instruments)
90
Physiologic Instruments voltage clamp/pulse generator model vcc600
(A) Kymographs of axonal EGFP-LC3+ vesicles in PPM1H KO iNeurons transiently expressing CFP (“Mock”) or ARF6 Q67L -CFP. Example AV traces are highlighted. (B) Directionality of AVs in PPM1H KO iNeurons transiently expressing CFP or ARF6 Q67L -CFP. Antero., anterograde; retro., retrograde; bidir., bidirectional; stat., stationary (mean ± SEM; n = 32-34 neurons from 4 independent experiments; ns, not significant, p>0.9652; *p<0.0410; two-way ANOVA with Sidak’s multiple comparisons test). (C-G) Pause number (C) and pause duration (D) of motile AVs, fraction of time paused (E) of all AVs, and directional reversals (F) and Δ run length (G) of motile AVs in PPM1H KO iNeurons transiently expressing CFP or ARF6 Q67L -CFP (mean ± SD for panel C and E; n = 63-95 motile AVs (C, D, F, G) and 77-134 total AVs (E) from 32-34 neurons from 4 independent experiments; ns, not significant, p= 0.638; *p= 0.0396; ***p<0.001; mixed effects model analysis). For panels C and E, scatter plot points indicate the means of four independent experiments. For panels D, F, and G, curve fits were generated using nonlinear regression (two phase decay). (H-I) Model: imbalance between ARF6 and pRABs disrupts axonal AV transport in conditions of <t>LRRK2</t> hyperactivity (H) Under physiologic conditions, ARF6 binds to JIP4, promoting JIP4 binding to dynactin while discouraging the JIP4-kinesin interaction. With active dynein/dynactin and inactive kinesin, AVs undergo smooth retrograde transport in WT neurons. (I) Under pathologic conditions of LRRK2 hyperactivity, increased phosphorylation of RAB GTPases on the AV membrane leads to increased recruitment of JIP4, due to specific binding of JIP4 by RABs in their phosphorylated state. This allows for recruitment and disinhibition of kinesin by JIP4. The presence of active dynein/dynactin and active kinesin on the same organelle results in an unproductive tug-of-war between microtubule-associated motors, leading to non-processive motility and pausing behavior of AVs.
Voltage Clamp/Pulse Generator Model Vcc600, supplied by Physiologic Instruments, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/repeated+measures+general+linear+model+sas+proc+glm/automatic+voltage+clamp+vcc+600/pmc02232471-59-17-22
Average 90 stars, based on 1 article reviews
voltage clamp/pulse generator model vcc600 - by Bioz Stars, 2026-09
90/100 stars
  Buy from Supplier
ocean general circulation model  (Meso Scale Diagnostics LLC)
90
Meso Scale Diagnostics LLC ocean general circulation model
(A) Kymographs of axonal EGFP-LC3+ vesicles in PPM1H KO iNeurons transiently expressing CFP (“Mock”) or ARF6 Q67L -CFP. Example AV traces are highlighted. (B) Directionality of AVs in PPM1H KO iNeurons transiently expressing CFP or ARF6 Q67L -CFP. Antero., anterograde; retro., retrograde; bidir., bidirectional; stat., stationary (mean ± SEM; n = 32-34 neurons from 4 independent experiments; ns, not significant, p>0.9652; *p<0.0410; two-way ANOVA with Sidak’s multiple comparisons test). (C-G) Pause number (C) and pause duration (D) of motile AVs, fraction of time paused (E) of all AVs, and directional reversals (F) and Δ run length (G) of motile AVs in PPM1H KO iNeurons transiently expressing CFP or ARF6 Q67L -CFP (mean ± SD for panel C and E; n = 63-95 motile AVs (C, D, F, G) and 77-134 total AVs (E) from 32-34 neurons from 4 independent experiments; ns, not significant, p= 0.638; *p= 0.0396; ***p<0.001; mixed effects model analysis). For panels C and E, scatter plot points indicate the means of four independent experiments. For panels D, F, and G, curve fits were generated using nonlinear regression (two phase decay). (H-I) Model: imbalance between ARF6 and pRABs disrupts axonal AV transport in conditions of <t>LRRK2</t> hyperactivity (H) Under physiologic conditions, ARF6 binds to JIP4, promoting JIP4 binding to dynactin while discouraging the JIP4-kinesin interaction. With active dynein/dynactin and inactive kinesin, AVs undergo smooth retrograde transport in WT neurons. (I) Under pathologic conditions of LRRK2 hyperactivity, increased phosphorylation of RAB GTPases on the AV membrane leads to increased recruitment of JIP4, due to specific binding of JIP4 by RABs in their phosphorylated state. This allows for recruitment and disinhibition of kinesin by JIP4. The presence of active dynein/dynactin and active kinesin on the same organelle results in an unproductive tug-of-war between microtubule-associated motors, leading to non-processive motility and pausing behavior of AVs.
Ocean General Circulation Model, supplied by Meso Scale Diagnostics LLC, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/repeated+measures+general+linear+model+sas+proc+glm/ocean+general+circulation+model/10__1175_slash_jpo___d___15___0024__1-4-7-35
Average 90 stars, based on 1 article reviews
ocean general circulation model - by Bioz Stars, 2026-09
90/100 stars
  Buy from Supplier
cirs model 040 general purpose multi-tissue ultrasound phantom  (CIRS Inc)
90
CIRS Inc cirs model 040 general purpose multi-tissue ultrasound phantom
(A) Kymographs of axonal EGFP-LC3+ vesicles in PPM1H KO iNeurons transiently expressing CFP (“Mock”) or ARF6 Q67L -CFP. Example AV traces are highlighted. (B) Directionality of AVs in PPM1H KO iNeurons transiently expressing CFP or ARF6 Q67L -CFP. Antero., anterograde; retro., retrograde; bidir., bidirectional; stat., stationary (mean ± SEM; n = 32-34 neurons from 4 independent experiments; ns, not significant, p>0.9652; *p<0.0410; two-way ANOVA with Sidak’s multiple comparisons test). (C-G) Pause number (C) and pause duration (D) of motile AVs, fraction of time paused (E) of all AVs, and directional reversals (F) and Δ run length (G) of motile AVs in PPM1H KO iNeurons transiently expressing CFP or ARF6 Q67L -CFP (mean ± SD for panel C and E; n = 63-95 motile AVs (C, D, F, G) and 77-134 total AVs (E) from 32-34 neurons from 4 independent experiments; ns, not significant, p= 0.638; *p= 0.0396; ***p<0.001; mixed effects model analysis). For panels C and E, scatter plot points indicate the means of four independent experiments. For panels D, F, and G, curve fits were generated using nonlinear regression (two phase decay). (H-I) Model: imbalance between ARF6 and pRABs disrupts axonal AV transport in conditions of <t>LRRK2</t> hyperactivity (H) Under physiologic conditions, ARF6 binds to JIP4, promoting JIP4 binding to dynactin while discouraging the JIP4-kinesin interaction. With active dynein/dynactin and inactive kinesin, AVs undergo smooth retrograde transport in WT neurons. (I) Under pathologic conditions of LRRK2 hyperactivity, increased phosphorylation of RAB GTPases on the AV membrane leads to increased recruitment of JIP4, due to specific binding of JIP4 by RABs in their phosphorylated state. This allows for recruitment and disinhibition of kinesin by JIP4. The presence of active dynein/dynactin and active kinesin on the same organelle results in an unproductive tug-of-war between microtubule-associated motors, leading to non-processive motility and pausing behavior of AVs.
Cirs Model 040 General Purpose Multi Tissue Ultrasound Phantom, supplied by CIRS Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/repeated+measures+general+linear+model+sas+proc+glm/ultrasound+phantom/pm24448754-45-6-14
Average 90 stars, based on 1 article reviews
cirs model 040 general purpose multi-tissue ultrasound phantom - by Bioz Stars, 2026-09
90/100 stars
  Buy from Supplier
reproducibility model source code  (MathWorks Inc)
93
MathWorks Inc reproducibility model source code
(A) Kymographs of axonal EGFP-LC3+ vesicles in PPM1H KO iNeurons transiently expressing CFP (“Mock”) or ARF6 Q67L -CFP. Example AV traces are highlighted. (B) Directionality of AVs in PPM1H KO iNeurons transiently expressing CFP or ARF6 Q67L -CFP. Antero., anterograde; retro., retrograde; bidir., bidirectional; stat., stationary (mean ± SEM; n = 32-34 neurons from 4 independent experiments; ns, not significant, p>0.9652; *p<0.0410; two-way ANOVA with Sidak’s multiple comparisons test). (C-G) Pause number (C) and pause duration (D) of motile AVs, fraction of time paused (E) of all AVs, and directional reversals (F) and Δ run length (G) of motile AVs in PPM1H KO iNeurons transiently expressing CFP or ARF6 Q67L -CFP (mean ± SD for panel C and E; n = 63-95 motile AVs (C, D, F, G) and 77-134 total AVs (E) from 32-34 neurons from 4 independent experiments; ns, not significant, p= 0.638; *p= 0.0396; ***p<0.001; mixed effects model analysis). For panels C and E, scatter plot points indicate the means of four independent experiments. For panels D, F, and G, curve fits were generated using nonlinear regression (two phase decay). (H-I) Model: imbalance between ARF6 and pRABs disrupts axonal AV transport in conditions of <t>LRRK2</t> hyperactivity (H) Under physiologic conditions, ARF6 binds to JIP4, promoting JIP4 binding to dynactin while discouraging the JIP4-kinesin interaction. With active dynein/dynactin and inactive kinesin, AVs undergo smooth retrograde transport in WT neurons. (I) Under pathologic conditions of LRRK2 hyperactivity, increased phosphorylation of RAB GTPases on the AV membrane leads to increased recruitment of JIP4, due to specific binding of JIP4 by RABs in their phosphorylated state. This allows for recruitment and disinhibition of kinesin by JIP4. The presence of active dynein/dynactin and active kinesin on the same organelle results in an unproductive tug-of-war between microtubule-associated motors, leading to non-processive motility and pausing behavior of AVs.
Reproducibility Model Source Code, supplied by MathWorks Inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/repeated+measures+general+linear+model+sas+proc+glm/MATLAB+Report+Generator/pmc08318289__pcbi__1008143__s006-2-6-20
Average 93 stars, based on 1 article reviews
reproducibility model source code - by Bioz Stars, 2026-09
93/100 stars
  Buy from Supplier
5th-generation ncar/penn state mesoscale model (mm5)  (Meso Scale Diagnostics LLC)
90
Meso Scale Diagnostics LLC 5th-generation ncar/penn state mesoscale model (mm5)
(A) Kymographs of axonal EGFP-LC3+ vesicles in PPM1H KO iNeurons transiently expressing CFP (“Mock”) or ARF6 Q67L -CFP. Example AV traces are highlighted. (B) Directionality of AVs in PPM1H KO iNeurons transiently expressing CFP or ARF6 Q67L -CFP. Antero., anterograde; retro., retrograde; bidir., bidirectional; stat., stationary (mean ± SEM; n = 32-34 neurons from 4 independent experiments; ns, not significant, p>0.9652; *p<0.0410; two-way ANOVA with Sidak’s multiple comparisons test). (C-G) Pause number (C) and pause duration (D) of motile AVs, fraction of time paused (E) of all AVs, and directional reversals (F) and Δ run length (G) of motile AVs in PPM1H KO iNeurons transiently expressing CFP or ARF6 Q67L -CFP (mean ± SD for panel C and E; n = 63-95 motile AVs (C, D, F, G) and 77-134 total AVs (E) from 32-34 neurons from 4 independent experiments; ns, not significant, p= 0.638; *p= 0.0396; ***p<0.001; mixed effects model analysis). For panels C and E, scatter plot points indicate the means of four independent experiments. For panels D, F, and G, curve fits were generated using nonlinear regression (two phase decay). (H-I) Model: imbalance between ARF6 and pRABs disrupts axonal AV transport in conditions of <t>LRRK2</t> hyperactivity (H) Under physiologic conditions, ARF6 binds to JIP4, promoting JIP4 binding to dynactin while discouraging the JIP4-kinesin interaction. With active dynein/dynactin and inactive kinesin, AVs undergo smooth retrograde transport in WT neurons. (I) Under pathologic conditions of LRRK2 hyperactivity, increased phosphorylation of RAB GTPases on the AV membrane leads to increased recruitment of JIP4, due to specific binding of JIP4 by RABs in their phosphorylated state. This allows for recruitment and disinhibition of kinesin by JIP4. The presence of active dynein/dynactin and active kinesin on the same organelle results in an unproductive tug-of-war between microtubule-associated motors, leading to non-processive motility and pausing behavior of AVs.
5th Generation Ncar/Penn State Mesoscale Model (Mm5), supplied by Meso Scale Diagnostics LLC, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/repeated+measures+general+linear+model+sas+proc+glm/fifth+generation+mesoscale+model/pm16568797-74-20-23
Average 90 stars, based on 1 article reviews
5th-generation ncar/penn state mesoscale model (mm5) - by Bioz Stars, 2026-09
90/100 stars
  Buy from Supplier
second-generation nirs device inspectra model 325  (Hutchinson Technology Inc)
90
Hutchinson Technology Inc second-generation nirs device inspectra model 325
<t> Near infrared spectroscopy </t> variables in patients with ESRD and in healthy volunteers
Second Generation Nirs Device Inspectra Model 325, supplied by Hutchinson Technology Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/repeated+measures+general+linear+model+sas+proc+glm/nirs+inspectra/pmc04467734-73-12-18
Average 90 stars, based on 1 article reviews
second-generation nirs device inspectra model 325 - by Bioz Stars, 2026-09
90/100 stars
  Buy from Supplier

Image Search Results


Tested WRF physics parametrization configurations. The CONUS physics suite, recommended by NCAR, was chosen for better reproduction of the PRISM-based precipitation and daily maximum and minimum air temperature for an arbitrarily test year, 2009.

Journal: Scientific Data

Article Title: Continental United States climate projections based on thermodynamic modification of historical weather

doi: 10.1038/s41597-023-02485-5

Figure Lengend Snippet: Tested WRF physics parametrization configurations. The CONUS physics suite, recommended by NCAR, was chosen for better reproduction of the PRISM-based precipitation and daily maximum and minimum air temperature for an arbitrarily test year, 2009.

Article Snippet: Surface Layer , Eta Similarity scheme , Fifth-Generation Penn State/NCAR Mesoscale Model (Revised MM5) , Fifth-Generation Penn State/NCAR Mesoscale Model (Revised MM5) .

Techniques: Aerosol

(A) Kymographs of axonal EGFP-LC3+ vesicles in PPM1H KO iNeurons transiently expressing CFP (“Mock”) or ARF6 Q67L -CFP. Example AV traces are highlighted. (B) Directionality of AVs in PPM1H KO iNeurons transiently expressing CFP or ARF6 Q67L -CFP. Antero., anterograde; retro., retrograde; bidir., bidirectional; stat., stationary (mean ± SEM; n = 32-34 neurons from 4 independent experiments; ns, not significant, p>0.9652; *p<0.0410; two-way ANOVA with Sidak’s multiple comparisons test). (C-G) Pause number (C) and pause duration (D) of motile AVs, fraction of time paused (E) of all AVs, and directional reversals (F) and Δ run length (G) of motile AVs in PPM1H KO iNeurons transiently expressing CFP or ARF6 Q67L -CFP (mean ± SD for panel C and E; n = 63-95 motile AVs (C, D, F, G) and 77-134 total AVs (E) from 32-34 neurons from 4 independent experiments; ns, not significant, p= 0.638; *p= 0.0396; ***p<0.001; mixed effects model analysis). For panels C and E, scatter plot points indicate the means of four independent experiments. For panels D, F, and G, curve fits were generated using nonlinear regression (two phase decay). (H-I) Model: imbalance between ARF6 and pRABs disrupts axonal AV transport in conditions of LRRK2 hyperactivity (H) Under physiologic conditions, ARF6 binds to JIP4, promoting JIP4 binding to dynactin while discouraging the JIP4-kinesin interaction. With active dynein/dynactin and inactive kinesin, AVs undergo smooth retrograde transport in WT neurons. (I) Under pathologic conditions of LRRK2 hyperactivity, increased phosphorylation of RAB GTPases on the AV membrane leads to increased recruitment of JIP4, due to specific binding of JIP4 by RABs in their phosphorylated state. This allows for recruitment and disinhibition of kinesin by JIP4. The presence of active dynein/dynactin and active kinesin on the same organelle results in an unproductive tug-of-war between microtubule-associated motors, leading to non-processive motility and pausing behavior of AVs.

Journal: bioRxiv

Article Title: Regulatory imbalance between LRRK2 kinase, PPM1H phosphatase, and ARF6 GTPase disrupts the axonal transport of autophagosomes

doi: 10.1101/2022.11.14.516471

Figure Lengend Snippet: (A) Kymographs of axonal EGFP-LC3+ vesicles in PPM1H KO iNeurons transiently expressing CFP (“Mock”) or ARF6 Q67L -CFP. Example AV traces are highlighted. (B) Directionality of AVs in PPM1H KO iNeurons transiently expressing CFP or ARF6 Q67L -CFP. Antero., anterograde; retro., retrograde; bidir., bidirectional; stat., stationary (mean ± SEM; n = 32-34 neurons from 4 independent experiments; ns, not significant, p>0.9652; *p<0.0410; two-way ANOVA with Sidak’s multiple comparisons test). (C-G) Pause number (C) and pause duration (D) of motile AVs, fraction of time paused (E) of all AVs, and directional reversals (F) and Δ run length (G) of motile AVs in PPM1H KO iNeurons transiently expressing CFP or ARF6 Q67L -CFP (mean ± SD for panel C and E; n = 63-95 motile AVs (C, D, F, G) and 77-134 total AVs (E) from 32-34 neurons from 4 independent experiments; ns, not significant, p= 0.638; *p= 0.0396; ***p<0.001; mixed effects model analysis). For panels C and E, scatter plot points indicate the means of four independent experiments. For panels D, F, and G, curve fits were generated using nonlinear regression (two phase decay). (H-I) Model: imbalance between ARF6 and pRABs disrupts axonal AV transport in conditions of LRRK2 hyperactivity (H) Under physiologic conditions, ARF6 binds to JIP4, promoting JIP4 binding to dynactin while discouraging the JIP4-kinesin interaction. With active dynein/dynactin and inactive kinesin, AVs undergo smooth retrograde transport in WT neurons. (I) Under pathologic conditions of LRRK2 hyperactivity, increased phosphorylation of RAB GTPases on the AV membrane leads to increased recruitment of JIP4, due to specific binding of JIP4 by RABs in their phosphorylated state. This allows for recruitment and disinhibition of kinesin by JIP4. The presence of active dynein/dynactin and active kinesin on the same organelle results in an unproductive tug-of-war between microtubule-associated motors, leading to non-processive motility and pausing behavior of AVs.

Article Snippet: Lrrk2 -p.G2019S KI mice (model #13940) and B6NTac mice (model #B6) were obtained from Taconic, Cambridge City, Indiana production site.

Techniques: Expressing, Generated, Binding Assay, Phospho-proteomics, Membrane

Near infrared spectroscopy variables in patients with ESRD and in healthy volunteers

Journal: International Journal of Nephrology and Renovascular Disease

Article Title: Changes in skeletal muscle microcirculation after a hemodialysis session correlates with adequacy of dialysis

doi: 10.2147/IJNRD.S68639

Figure Lengend Snippet: Near infrared spectroscopy variables in patients with ESRD and in healthy volunteers

Article Snippet: In the present study, thenar StO 2 was measured non-invasively using a second-generation NIRS device (Inspectra Model 325, Hutchinson Technology, McLeod County, MN, USA).

Techniques: Spectroscopy